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ceilings, and floors should be smooth, impermeable to liquids and resistant to the
chemicals and disinfectants normally used in the laboratory. Floors should be monolithic
and slip-resistant. Consideration should be given to the use of coved floor coverings.
Penetrations in floors, walls, and ceiling surfaces are sealed. Openings such as around
ducts and the spaces between doors and frames are capable of being sealed to facilitate
Bench tops are impervious to water and are resistant to moderate heat and the organic
solvents, acids, alkalis, arid those chemicals used to decontaminate the work surfaces
and equipment.
Laboratory furniture is capable of supporting anticipated loading and uses. Spaces
between benches, cabinets, and equipment are accessible for cleaning. Chairs and other
furniture used in laboratory work should be covered with a non-fabric material that can be
easily decontaminated.
All windows in the laboratory are closed and sealed.
A method for decontamination all laboratory wastes is available in the facility and utilized,
preferably within the laboratory (i.e., autoclave chemical disinfections, incineration, or
other approved decontamination method). Consideration should be given to means of
decontaminating equipment. If waste is transported out of the laboratory, it should be
properly sealed and not transported in public corridors.
Biological safety cabinets are required and are located away from doors, from room
supply louvers, and from heavily traveled laboratory areas.
A ducted exhaust air ventilation system is provided. This system creates directional
airflow, which draws air into the laboratory from "clean" areas and toward "contaminated"
areas. The exhaust air is not re-circulated to any other area of the building. Filtration and
other treatments of the exhaust air are not required, but may be considered based on site
requirements, and specific agent manipulations and use conditions. The outside exhaust
must be dispersed away from occupied areas and air intakes, or the exhaust must be
HEPA-filtered. Laboratory personnel must verify that the direction of the air flow (into the
laboratory) is proper. It is recommended that a visual monitoring device that indicates and
confirms directional inward airflow be provided at the laboratory entry. Consideration
should be given to installing an HVAC control system to prevent sustained positive
pressurization of the laboratory. Audible alarms should be considered to notify personnel
of HVAC system failure.
HEPA-filtered exhaust air from Class II biological safety cabinet can be re-circulated into
the laboratory, if the cabinet is tested and certified at least annually. When exhaust air
from Class II safety cabinet is to be discharged to the outside through the building
exhaust air system, the cabinet must be connected in a manner that avoids any
interference with the air balance of the cabinets or the building exhaust system (i.e. an air
gap between the cabinet exhaust and the exhaust duct). When Class III biological safety
cabinets are used, they should be directly connected to the exhaust system. If the Class
III cabinets are connected to the supply system, it is done in a manner that prevents
positive pressurization of the cabinets.
Continuous flow centrifuges or other equipment that may produce aerosols are contained
in devices that exhaust air through HEPA filters before discharge into the laboratory.
These HEPA systems are tested at least annually. Alternatively, the exhaust from such
equipment may be vented to the outside if it is dispersed away from occupied areas and
air intakes.
Vacuum lines are protected with liquid disinfectant traps and HEPA filters, or their
equivalent. Filters must be replaced as needed. An alternative is to use portable vacuum
pumps (also properly protected with traps and filters).
An eyewash station is readily available inside the laboratory.
Illumination is adequate for all activities, avoiding reflections and glare that could impede


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